MANOHAR SINGH BISHT
PhD Scholar (PMRF)
KEY PUBLICATIONS
1. Mahajan, S., Bisht, M. S., Chakraborty, A., & Sharma, V. K. (2023). Genome of Phyllanthus emblica: the medicinal plant Amla with super antioxidant properties. bioRxiv, 2023-05.​
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2. Chakraborty, A., Bisht, M. S., Saxena, R., Mahajan, S., Pulikkan, J., & Sharma, V. K. (2023). Genome sequencing and de novo and reference-based genome assemblies of Bos indicus breeds. Genes & Genomics, 1-10.
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3. Chakraborty, A., Mahajan, S., Bisht, M. S., & Sharma, V. K. (2022). Genome sequencing and comparative analysis of Ficus benghalensis and Ficus religiosa species reveal evolutionary mechanisms of longevity. Iscience, 25(10), 105100. https://doi.org/10.1016/j.isci.2022.105100
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Genome sequencing of National Tree of India; Banyan Tree and Peepal Tree -Defining the evolutionary mechanism of longevity
Indian Banyan Tree (Ficus benghalensis) and Peepal (Ficus religiosa) are plant species from Moraceae plant family well-known for their long lifespan, religious significance, and medicinal properties. We performed the first draft genome sequencing and analysis of Ficus benghalensis and Ficus religiosa, belonging to Moraceae plant family, using 10x Genomics linked-read and Oxford Nanopore sequencing technologies. Comparative evolutionary analysis performed across 20 species including six short-lived and long-lived Ficus species revealed evolution in genes involved in key cellular mechanisms associated with prolonged survival including phytohormones signalling, senescence, disease resistance genes and their high expression, and abiotic stress tolerance, which provide new genomic insights into the mechanisms that confer longevity and suggest that longevity is a multifaceted phenomenon.
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Genome sequencing of Indigenous cattle breeds
Bos indicus is a domestic cattle species with many indigenous breeds found in India and is important for dairy, draught work, and other household activities. We performed the whole genome sequencing to construct reference-based genome assemblies of four B. indicus breeds; Ongole, Kasargod Dwarf, Kasargod Kapila, and Vechur, of which Vechur is known as the smallest cow of the world. We also report the first de novo genome assemblies of these native B. indicus breeds. Genomic analysis helped to identify the distinct bovine phenotypic characteristics-related and other biological process-related genes in this species compared to B. taurus, and to gain comparative genomic insights between the dwarf and non-dwarf breeds of this species.
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Past Sequenced Genomes from the MetaBioSys
Plant Genomes
Animal Genomes
